Highranger plus 100bp dna ladder 100bp 0bp analyzing dna over a wide range of sizes. This product is available in quickload purple format enjoy the convenience of a readytoload format. This marker can be used with either agarose or polyacrylamide gels. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to. Sigmaaldrich offers a number of directload 1 kb dna ladder products. First aid measures general information this solution is not. The invitrogen 1kb plus dna ladder is designed for sizing and quantification of double stranded dna on 0. Dna isolation protocol that yields highly concentrated plasmid dna. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. All of these form less than 1 % wv of the final mixture, so they are not listed in the table above. The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp. The dna 1 kb ladder is suitable for size determination of doublestranded dna using dna electrophoresis. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as.
Product identifier 1 kb dna ladder or ruler trade namebrand invitrogen1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable cas number mixture, not applicable ec number mixture, not applicable relevant identified uses this product is a laboratory preparation for. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. Neb also offers various conventional doublestranded dna molecular weight markers with sizes up to 23 kb and markers for pulsedfield gel electrophoresis pfge ranging from 1. Available on gsava contract for federal government customers. How can i prepare working solution with 1 kb dna ladder. For the convenience, the amounts of the 500, 1kbp and 5kbp fragments are adjusted to show greater intensities.
Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. The 1 kb and 3 kb bands contain more dna to provide internal orientation. The ladder is prepared from a plasmid containing repeats of a 1018 bp dna fragment. Egel 1 kb plus dna ladder consists of 18 individual chromatographypurified dna fragments and has a reference band at 1,500 bp for easy orientation. For loading your gel you have to add a loading buffer to the marker. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp.
For easy reference on agarose gels, the 5kb band is two to threetimes brighter than the other bands in the ladder. The ladder is composed of fifteen chromatographypurified individual dna fragments in base pairs. Thermo scientific generuler 1 kb plus dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 75 bp to 20,000 bp on agarose gels. Invitrogen 1 kb plus dna ladder 1,000g life sciences. Invitrogen egel 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Egel 1 kb plus dna ladder thermo fisher scientific. If samples need to be diluted, use te or other buffer of minimal ionic strength. The ladder is premixed with gel load buffer color dyes with varying mobility on an agarose gel for direct transfer to the gel. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. Br0800101 100 lanes 500 l 1 kb dna ladder rtu 1 ml dna loading dye component composition 1 kb dna ladder rtu readyto use dna in 10 mm trishcl ph 8.
The dna ladder consists of 15 dna fragments and is provided with 6x tritrack dna loading dye. Do not heat the trackit 1kb plus dna ladder before loading. The 1kb dna extension ladder consists of 8 fragments ranging in length from 1,018 to 8,144bp in 1,018bp increments, as well as bands of 506bp, 517bp, 1,636bp, 10kb, 15kb, 20kb, and 40kb. The 1 kb plus dna ladder consists of 18 individual chromatographypurified dna fragments ranging in size from 100 bp to 15,000 bp. Neb dna ladders are available in standard formats and in a variety of convenient, readytoload formats. In addition to these 12 bands, the ladder contains vector dna fragments that range from 75 to 1636 bp. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. Tridye 1 kb plus dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. Use the 1 kb plus dna ladder is to size linear doublestranded dna fragments from 100 bp to 12 kb. Invitrogen 1kb dna extension ladder 100ug nucleic acid. A reference band at 1,500 bp is included for easy orientation.
Invitrogen 1 kb plus dna ladder nondistribution product as customer accommodation. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. A transcription activatorlike effector toolbox for. Invitrogen egel 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15000 bp. Invitrogen 1 kb plus dna ladder 10787026 print share invitrogen 1 kb plus dna ladder. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp. Premixed with loading buffer ready to load room temperature stability high dna mass ladder 4 llane. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. Divide n1n18 into subsequences of length 6 n1n2n3n4n5n6, n7n8n9 n10n11n12 and. Generuler 1 kb plus dna ladder thermo fisher scientific. Ultraranger 1kb dna ladder 300bp 24000bp size determination of digested dna with high molecular weight more 100bp and 1 kb ladders are offered from our partner biovision.
The ladder consists of nine bands in even increments 0. For easy reference on agarose gels, the 5kb band is two. The product is supplied in a solution of 10 mm trishcl, ph 8. This visual guide allows you to easily compare the differences between dna ladders offered at goldbio in terms of size. Invitrogen 10787026 view more versions of this product. Plus dna ladder if you need size markers for fragments between 100 bp and 12 kb. View more information about our dna stains technology. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. Catalog number size important guidelines 10787018 250 g.
Nucleic acid ladder is used as a size standard in agarose gel electrophoresis. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and. This marker is ideal for the size determination of digested dna. Dna marker blue bp 1kb dna ladder readytouse dna marker bp 1kb dna ladder is designed for use as molecular weight standard for agarose gel electrophoresis features. The invitrogen 1 kb plus dna ladder thermo fisher scientific. Price pricing info supplier page view company product page. Alexa 647labeled antimouse antibody from invitrogen carlsbad. Here we describe an improved protocol for rapid construction of. Powerful tool for estimating molecular weight of linear, doublestranded dna fragments. The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer. The digested dna includes fragments ranging from 0. Thermo scientific generuler 1 kb plus dna ladder is designed for sizing and quantification of dna fragments in agarose gels.
The 8 kb and 14 kb plasmids have the same backbone, pegfpn1. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. Generuler 1 kb plus dna ladder, readytouse thermo fisher.
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